Cigarette smoke could alter shape of heart Nov 14, 2008
Heart tissue was examined under the microscope and by Western blot analysis, used to detect specific proteins in tissue samples. The results showed exposure to cigarette smoke was associated with significant changes in the shape of the left ventricle, the heart's main pumping chamber, and an increase in the levels of the activated forms of the enzymes in the heart muscle. (EurekAlert!)
Spontaneous transformation of human granulosa cell tumours into an aggressive phenotype: a metastasis model cell line Nov 4, 2008
The expression level of tumour markers in KGN cells at different passages was then determined by Western blot analysis. Finally, the growth and metastasis of KGN cells injected subcutaneously (s. (BioMed Central)
Rapid Screening Tests for HIV Oct 20, 2008
Other HIV tests, like the ELISA or Western Blot Test, take up to two weeks before patients receive their test results. This time delay means that patients suffer anxiety for weeks before receiving their results and other patients never return to the clinic to pick up the results of their HIV test. (Suite101.com)
Researchers discover that growing up too fast may mean dying young in honey bees Sep 25, 2008
Antioxidant proteins Hsp70 and catalase were measured in head and thorax tissues using western blot. Total antioxidant capacity of the tissues was measured as the ability of homogenate to inhibit the oxidation of ABTS (2,20-azino-di-[3-ethylbenzthiazolinesulphonate]) to ABTS+ relative to trolox standards. (EurekAlert!)
A human monoclonal autoantibody to breast cancer identifies the PDZ domain containing protein GIPC1 as a novel breast cancer-associated antigen Aug 25, 2008
The current study employs immunocytochemistry, immunohistochemistry, Western blot analysis as well as Northern blots, Scatchard binding studies and finally SEREX analysis for target antigen identification. Results. (BioMed Central)
Native human autoantibodies targeting GIPC1 identify differential expression in malignant tumors of the breast and ovary Aug 25, 2008
The current study employs cELISA, flow cytometry, Western blot analysis as well as immunocytochemistry, and immunohistochemistry. Data is analyzed statistically with the Fisher one-tail and two-tail tests for two independent samples. (BioMed Central)
Proteomic analysis identifies MMP-9, DJ-1 and A1BG as overexpressed proteins in pancreatic juice from pancreatic ductal adenocarcinoma patients Aug 16, 2008
Of the identified differently expressed proteins, three up-regulated proteins in pancreatic cancer juice, matrix metalloproteinase-9 (MMP-9), oncogene DJ1 (DJ-1) and alpha-1B-glycoprotein precursor (A1BG), were selected for validation by Western blot and immunohistochemistry ... Increased MMP-9, DJ-1 and A1BG expression in cancerous pancreatic juice were confirmed by Western blot. (BioMed Central)
Summary for Patients Aug 5, 2008
These tests included a serum enzyme-linked immunoassay, a single serum Western blot test, CD4 count, and plasma HIV-1 RNA measurement. Results of the confirmatory tests were not available the day of the emergency department visit. (Annals of Internal Medicine)
Connexin-43 upregulation promotes tumor cell adhesion Jul 23, 2008
High-resolution immunofluorescence microscopy and Western blot analysis was performed using a connexin-43 monoclonal antibody. Calcein Orange Red AM dye transfer by fluorescence imaging was used to evaluate the gap junction function. (BioMed Central)
Incorrect Diagnosis of Lyme Disease Jul 21, 2008
Positive results on both the ELISA test and Western Blot test are required by the Center for Disease Control (CDC) in order to report a case of Lyme disease ... The Western Blot test is far more accurate because it looks for proteins shed from the bacteria and the blebs ... IDSA guidelines restrict physicians from ordering the Western Blot test before the ELISA test or if the ELISA test results are negative. (Suite101.com)
Clinical study shows biological and clinical activity in relapsed leukemia patients Jun 5, 2008
Caspases -8 and -9 were activated in the CLL cells from all six evaluable patients, as measured by western blot analysis. DNA fragmentation in patients' CLL cells also was observed, strengthening the evidence that GCS-100 induces apoptosis. (EurekAlert!)
'PLoS ONE' STUDY: A Low Dose of Dietary Resveratrol Partially Mimics Caloric Restriction and Retards Aging Parameters in Mice Jun 4, 2008
GLUT4 protein abundance was determined in soleus and EDL muscles by western blotting (n = 16 muscles per group) ... (A) SIRT1 levels in liver, skeletal muscle and brain (n = at least four animals per group) were determined by Western blot analysis ... GLUT4 protein abundance was determined in soleus and EDL muscles by western blotting (n = 16 muscles per group). (USA Today -- Tech)
Expression of TRPC6 channels in human epithelial breast cancer cells May 2, 2008
Molecular (Western blot and RT-PCR), and immunohistochemical techniques were used to investigate TRPC6 expression. To investigate the channel activity in both MCF-7 cells and hBCE we used electrophysiological technique (whole cell patch clamp configuration). (BioMed Central)
Nanopolymers improve delivery of exon skipping oligonucleotides and concomitant dystrophin expression in skeletal muscle of mdx mice Apr 3, 2008
Surprisingly, using the same injection and dosing regimen, we found no significant difference in dystrophin expression by western blot between the NG-PEI2K-PEG550, CG-PEI2K-PEG5K, and non-functionalized PEI2K-PEG550 copolymers. Dose-response experiments using the CG-PEI2K-PEG5K copolymer with total ESO ranging from 3-60 micrograms yielded a maximum of about 15% dystrophin expression. (BioMed Central)
Neurologic Benefit of Lyme Therapy Short-Lived Mar 27, 2008
Fallon, from Columbia University in New York, and colleagues investigated the benefits of 10 weeks of intravenous ceftriaxone (trade name Rocephin) in patients with previously treated Lyme disease who continued to show memory impairments and tested positive for Lyme disease using a Western blot. A total of 37 patients were randomly assigned to receive intravenous ceftriaxone or placebo (saline solution) for 10 weeks. (MEDLINEplus)
Aminopeptidase N (APN)/CD13 inhibitor, Ubenimex, enhances radiation sensitivity in human cervical cancer Mar 19, 2008
Molecular changes of apoptosis were determined by Western blot. Apoptosis was evaluated by Annexin-V PI staining (flow cytometry analysis) and the Tunel method. (BioMed Central)
Autism's origins: Mother's antibody production may affect fetal brain Feb 26, 2008
To test their hypothesis, the research team used a technique called immunoblotting (or Western blot technology), in which antibodies derived from blood samples are exposed to adult and fetal brain tissue to check whether the antibodies recognize and react against specific brain proteins. Comparing the antibody-brain interaction in samples obtained from 100 mothers of autistic children and 100 mothers of children without autism, researchers found either stronger reactivity or more areas of... (EurekAlert!)
Berberine enhances inhibition of glioma tumor cell migration and invasiveness mediated by arsenic trioxide Feb 25, 2008
Zymography and Western blot analyses provided information on the effect of As2O3 and berberine on the intracellular translocation and activation of protein kinase C (PKC), and some PKC-related downstream factors. Most assays were performed three times, independently, and data were analyzed using ANOVA.. (BioMed Central)
Antitumor activity and mechanisms of action of total glycosides from aerial part of Cimicifuga dahurica targeted against hepatoma Jan 1, 2008
Morphology observation, Annexin V-FITC/PI staining, cell cycle analysis and western blot were used to further elucidate the cytotoxic mechanism of TGA. Implanted mouse H22 hepatoma model was used to demonstrate the tumor growth inhibitory activity of TGA in vivo. Results. (BioMed Central)
Fenretinide-induced apoptosis of Huh-7 hepatocellular carcinoma is retinoic acid receptor beta dependent Jan 1, 2008
Apoptosis was determined by caspase-3 cleavage with western blot, DNA double-strand breaks with TUNEL assay, and phosphatidylserine translocation with flow cytometry analysis. Gene expression of nuclear receptors was assessed by real-time PCR. Transactivation assay and chromatin immunoprecipitation (ChIP) were conducted to evaluate the activation of retinoid X receptor alpha (RXRalpha)/retinoic acid receptor beta (RARbeta) pathway by fenretinide. (BioMed Central)
HIV testing: accrediting laboratories Dec 7, 2007
Patients are also advised to confirm their positive status through a Western Blot test. But for all the efforts to get themselves audited by TNSACS, the private laboratories stand to gain much. (Hindu)
Overtaken By Hype Dec 1, 2007
In 1987, no one wanted to hear about AIDS. Twenty years later, the wheel has come full circle. The spokes, however, are completely different. (India Times, India)
Stem cells model disease Nov 15, 2007
Using RT-PCR and Western blot analysis, they found that the undifferentiated stem cells normally transcribed the Fragile X gene and produced the FMR protein. But when the cells were induced to differentiate, both the FMR1 mRNA and FMR protein levels dropped significantly, suggesting that differentiation is necessary to trigger the silencing mutation and cause the symptoms of the disease. (The Scientist)
Cognitive Impairment Due To Chronic Lyme Disease Can Be Treated Oct 13, 2007
All patients had to meet criteria for memory impairment at the start of the study and they were also required to have a positive IgG Western blot for Lyme disease at study entry. Key findings from the Neurology paper are as follows. (Science Daily)
Proteome analysis of human gastric cardia adenocarcinoma by laser capture microdissection Oct 11, 2007
In addition, expressions of HSP27, 60, and Prx-2 in GCA specimens were further confirmed by immunohistochemical and western blot analyses. Conclusions. (BioMed Central)
Differential regulation of iron chelator-induced IL-8 synthesis via MAP kinase and NF-kappaB in immortalized and malignant oral keratinocytes Sep 13, 2007
Phosphorylation and degradation of the I-kappa B were analyized by Western blot. Results. (BioMed Central)
OUR OPINION: State board makes right call on Senate proposal Sep 6, 2007
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Summer-school scientists Aug 18, 2007
On a recent afternoon he showed a visitor the fluorescent microscope and Western blot machine he had learned to use from his mentor, research fellow Kulandayan Subramanian. "When I first came to the lab, I was freaking out -- I saw all these biohazard signs and I was scared to touch anything," he said. (Boston Globe)
Former UPenn postdoc faked images Aug 8, 2007
The ORI investigation, published in the Federal Register on July 16, concluded that Roovers had faked 19 panels of Western blot data, which appeared on 11 figures in 3 studies published between 2001 and 2003, Dahlberg said ... The editors "found various pieces of Western blots cut and pasted either within a figure or between figures," said Birnbaum, who said. (The Scientist)
Bernese Mountain Dogs Prone To Infection By Lyme Disease-related Bacteria Jul 16, 2007
The team used an enzyme-linked immunosorbent assay (ELISA) as well as a Western blot test to see whether the dogs had developed antibodies against B. burgdorferi. Of the Bernese Mountain Dogs, 58 percent had developed antibodies against B. burgdorferi, compared with only 15 percent of the control dogs. (Science Daily)
Bernese Mountain Dogs susceptible to spirochete infection Jul 13, 2007
Seroprevalence of B. burgdorferi was assessed by ELISA and Western blot and was 58% in Bernese Mountain Dogs compared to 15% in control dogs. This difference was significant. (BioMed Central)
National HIV Testing Day --- June 27, 2007 Jun 27, 2007
Patients who had positive rapid tests were given risk-reduction counseling and asked to provide a whole blood or oral specimen for confirmatory testing by Western blot. Testing procedures for the three sites differed by the location within the ED where HIV testing was offered and by the personnel responsible for testing and counseling. (Food Consumer)
Adult stem cell figure retracted Jun 14, 2007
In March, the magazine reported that two Western blot figures within a 2001 study in Blood journal resembled each other, despite the fact that they were describing different proteins. that the blots were similar to a blot used to describe yet a third protein type in a submitted by Verfaillie and others. (Scientist)
The 2007 Life Sciences Industry Awards Apr 18, 2007
The sales representatives "come into the lab and sell not just an instrument but a suite of products." James Smyth, a researcher at the University of California, San Francisco, uses Bio-Rad's VersaDoc MP 4000 image analysis system for DNA gel imaging and Western blotting analysis. "We can do multiplexed Western blotting," says Smyth ... "This one can do chemiluminescence for Western blot," she says, with additional features such as the ability to image fluorescent and colorimetric samples. (The Scientist)
Autoimmune Hearing Loss Mar 31, 2007
Sensorineural Hearing Loss in Autoimmune Disease. In 1979, the physician McCabe first described a group of patients with sudden sensorineural hearing loss (related to the inner ear) who improved after being treated with corticosteroids. (Suite101.com)
Identification and characterization of bovine regulator of telomere length elongation helicase gene (RTEL): molecular cloning, expression distribution, splice variants and DNA methylation profile Mar 6, 2007
Four splice variants were identified within the transcripts in both the coding and 5-untranslated regions; Western blot revealed that the most abundant splice variant SV-1 was translated to a truncated isoform of RTEL. The different 5'UTRs imply alternative transcription start sites in the promoter; Bovine RTEL was transcribed at the blastocyst stage, and expression levels were highest in adult testis, liver and ovary. DNA methylation analysis of tissues that differed significantly in expression... (BioMed Central)
A recombinase system facilitates cloning of expression cassettes in the ciliate Tetrahymena thermophila Mar 1, 2007
The functional expression of these genes was demonstrated by western blot analysis with MSP1 specific antibodies and by a blasticidin growing assay. Conclusions. (BioMed Central)
Degradation of the LDL receptors by PCSK9 is not mediated by a secreted protein acted upon by PCSK9 extracellularly Mar 1, 2007
Incubation of a secreted, truncated LDLR without cytoplasmic and transmembrane domains, as well as membrane fractions from HepG2 cells, with conditioned medium containing PCSK9, did not reduce the amount of LDLR as determined by western blot analysis. Thus, the LDLR is not degraded by PCSK9 on the cell surface. (BioMed Central)
Nicotine: The Link Between Cigarette Smoking And Kidney Disease? Jan 31, 2007
Western blots were performed after the cells fasted and had been stimulated for 24 hours with nicotine (10-7) ... Blots were incubated overnight with one of nine antibodies, which included mouse anti- 2-nAChR mAb s, anti- 3-nAChR mAb s, or anti- 4-nAChR mAb s. Fibronectin mRNA expression was determined by real time PCR and protein expression was measured by western blot. (Science Daily)
Nicotine: The link between cigarette smoking and kidney disease progression? Jan 30, 2007
Western blots were performed after the cells fasted and had been stimulated for 24 hours with nicotine (10-7) ... Fibronectin mRNA expression was determined by real time PCR and protein expression was measured by western blot. (EurekAlert!)
Lack of Association between Antimyelin Antibodies and Progression to Multiple Sclerosis Jan 25, 2007
Antibodies were assessed by Western blot analysis at baseline, and the results compared with the time and rate of progression to clinically definite multiple sclerosis or a diagnosis of multiple sclerosis as defined by an international panel (the McDonald criteria) ... Conclusions Serum antibodies against MOG and MBP, as detected by Western blot analysis, are not associated with an increased risk of progression to clinically definite multiple sclerosis in patients who have had a clinically... (New England Journal of Medicine)
Discovering biomarkers from gene expression data for predicting cancer subgroups usingneural networks and relational fuzzy clustering Jan 6, 2007
Our findings suggest the possibility of developing specialized microarray chips or use of real-time qPCR assays or antibody based methods such as ELISA and western blot analysis for an easy and low cost diagnosis of the subgroups. 1999-2007 BioMed Central Ltd unless otherwise stated. (BioMed Central)
