For Sale: Human Eggs Become a Research Commodity Nov 18, 2009
Instead scientists have primarily relied on eggs left over from in vitro fertilization (IVF) procedures. The secondhand supply, however, is small, and some question the quality of these eggs. (Scientific American)
Why the U.S. Gets a D on Preterm Birth Rates Nov 18, 2009
The rise in assisted reproductive technologies, such as in vitro fertilization, is another; these techniques are associated with both an increased risk of multiples as well as a higher risk of premature delivery, even of singletons. Status of health insurance matters as well. (Time.com)
Bee products prevent VEGF-induced angiogenesis in human umbilical vein endothelial cells Nov 17, 2009
In an in vitro tube formation assay, HUVECs and fibroblast cells were incubated for 14 days with VEGF and various concentrations of bee products [RJ, ethanol extract of bee pollen, ethanol extract of Chinese red propolis and its constituent, caffeic acid phenethyl ester (CAPE). To clarify the mechanism of in vitro angiogenesis, HUVEC proliferation and migration were induced by VEGF with or without various concentrations of RJ, bee pollen, Chinese red propolis, and CAPE.. (BioMed Central)
Adult Cell Expansion Without Stem Cells? Nov 17, 2009
The team of CNRS and INSERM researchers led by Michael Sieweke has nonetheless been able to generate mouse macrophages in vitro thanks to a genetic modification that inactivates the transcription factors(2) called MafB and c-Maf. Furthermore, once reinjected into the animal, these modified cells behave normally: they do not form a tumor, and they perfectly perform the tasks expected of an adult macrophage, such as ingesting bacteria and secreting the chemical agents capable of killing them. (Science Daily)
Bishops' Leader: Church Needs Say in Health Care Nov 17, 2009
The statement also explains in question-and-answer format the church's opposition to reproductive technologies such as in vitro fertilization that replace the "self-giving love" of a husband and wife. The bishops will also take up a proposed update of their "Ethical and Religious Directives for Catholic Health Care Services." The draft revision addresses nutrition and hydration for patients in a persistent vegetative state an issue that caused a national uproar in the case of Terri Schiavo, the... (Fox News)
Gourmet Cooking With Nut Oils Nov 15, 2009
Kris-Etherton, et al. Low Fat and High Monounsaturated Fat Diets Decrease Human Low Density Lipoprotein Oxidative Susceptibility in Vitro. Journal of Nutrition. (Suite101.com)
Give thanks you’re not related Nov 14, 2009
Nadya Suleman Last January in California, Suleman, 33, gave birth to eight children conceived through in vitro fertilization. An unemployed single mother on public assistance, Suleman had six more kids at home, a situation insufficiently alarming to keep the media from jumping all over the Octomom saga with both Manolo Blahniks. (Boston Globe)
Bacterial cotranslational targeting by FtsY Nov 14, 2009
In the current study we have determined the contribution of soluble FtsY to co-translational targeting in vitro and have re-analysed the localization of FtsY in vivo by fluorescence microscopy. Our data show that FtsY can bind to SRP-ribosome nascent chains (RNCs) in the absence of membranes. (BioMed Central)
Squeezing Light Into Much Tighter Spaces Than Previously Believed Possible Nov 14, 2009
"By being able to use our optical fibers as sensors -- rather than just using them as pipes to transmit light -- we can develop tools that, for example, could easily detect the presence of a flu virus at an airport; could help IVF (in vitro fertilization) specialists to determine which egg should be chosen for fertilization; could gauge the safety of drinking water; or could alert maintenance crews to corrosion occurring in the structure of an aircraft," says Professor Tanya Monro, Federation... (Science Daily)
Tosoh Releases First-Half Consolidated Results for Fiscal 2010 Nov 13, 2009
Our shipments of packing materials in Japan rose, and our shipments of in vitro diagnostic reagents were equally firm. The number of automated immunoassay analyzers that we sold domestically and abroad fell. (JCN Network, Japan)
Ivax Diagnostics Q3 revenue, income slip Nov 13, 2009
Charles Struby, president and CEO of the Miami-based company (AMEX: IVD), said in a news release that it has been a challenging year, but that it was taking steps to improve operations and competitiveness while increasing our footprint in the global in vitro diagnostics market. He said the company is planning for the launch of a new products line stemming from the distribution agreement it signed in July with. (South Florida Business Journal, FL)
The Palin Persuasion Nov 13, 2009
The mandates for insurance that state governments impose--guaranteed coverage for hair plugs or in vitro fertilization, for instance--increase prices. And since an individual cannot shop for insurance across state lines, a young, healthy person in mandate-manic New York cannot buy a low premium, high-deductible plan on offer in lightly regulated Utah. (CBS News -- Opinion)
Celine Dion will keep trying to have a baby Nov 12, 2009
Singers in vitro treatments in August, October were unsuccessful ... Forty-one-year-old Dion's doctor announced that she was pregnant via in vitro fertilization very early in the process, and it is now revealed that the embryos transferred in August and October were unsuccessful. (MSNBC -- News)
False Alarm; Celine Dion Not Pregnant After All Nov 12, 2009
Dion's U.S.-based publicist, Kim Jakwerth, said Wednesday the couple's doctor had initially confirmed a pregnancy in August through in vitro fertilization ... Dion, 41, and Rene Angelil, 67, have an 8-year-old son Rene-Charles who was conceived through in vitro fertilization. (KWTX.com, TX)
UBI gets cash boost Nov 10, 2009
Universal Biosensors is focused on the development, manufacture and commercialisation of a range of in vitro diagnostic tests for point-of care use. The company has had an ongoing relationship with LifeScan since 2001. (Sydney Morning Herald -- Business)
Stem Cells May Offer Alternative to Lung Transplants Nov 10, 2009
Human embryonic stem cells "have the capacity to differentiate in vivo and in vitro into cells from all three germ lineages, making them particularly important in developmental biology, regenerative medicine and in vitro pharmacological studies," Van Haute said. The study was published Nov. 5 in the journal Respiratory Research. (MEDLINEplus)
Frozen Embryo Adoptions Allow Mom To Carry Child Nov 9, 2009
Through the process of in vitro fertilization, Elizabeth's biological parents produced many embryos, with the unused embryos frozen. The parents had three options: dispose of the remaining embryos, donate them to science or put them up for adoption. (TheSanDiegoChannel.com, CA)
Lung Tissue Generated From Human Embryonic Stem Cells Nov 6, 2009
Van Haute continues, "hESC have the capacity to differentiate in vivo and in vitro into cells from all three germ lineages, making them particularly important in developmental biology, regenerative medicine and in vitro pharmacological studies. hESC lines carrying a monogenic disease affecting the lung, such as cystic fibrosis, are available. This novel technique can be used on these affected hESC lines and provide researchers with putatively clinically relevant tools to develop in vitro models... (Science Daily)
Spinal Cord Regeneration: Scar-degrading Enzyme Nov 5, 2009
The result -- the enzyme's activity was stabilized at internal body temperature for up to four weeks during in vitro tests. The researchers then used a lipid microtube-hydrogel scaffold system to deliver the thermostabilized enzymes into animals via a single injection. (Science Daily)
Testosterone and Spermatogenesis Nov 5, 2009
The mutant luteinizing hormone had a low level of partial activity in vitro. We concluded that the residual luteinizing hormone activity, resulting in the expression of steroidogenic enzymes in few mature Leydig cells producing small amounts of intratesticular testosterone (20. (New England Journal of Medicine)
MediciNova Announces Initiation of a Phase Ib Clinical Trial for MN-221 in Patients With Stable, Moderate to Severe Chronic Obstructive Pulmonary Disease Nov 4, 2009
Preclinical testing in vitro and in vivo shows MN-221 to be more selective for the beta2-adrenergic receptor than other beta2-adrenergic receptor agonists commonly used for these asthma attacks. This improved selectivity, coupled with its partial agonist activity at beta1-adrenergic receptors, may result in fewer cardiovascular side effects than are commonly observed with these other agents. (Primezone Releases)
* Taiwan News Quick Take Nov 4, 2009
HEALTH First in vitro baby prospers. The nations first baby born through in vitro fertilization has grown into a handsome man who, at 24, is now studying biomedicine at a graduate institute at National Taiwan University, medical sources said ... The baby was born in 1985 at Veterans General Hospital in Taipei, seven years after the worlds first in vitro baby was delivered in the UK. Although Japan cultivated Asias first in vitro baby, the infant died slightly more than a year later,... (Taipei Times, Taiwan -- World Business)
An option for public: less government, more choice Nov 4, 2009
Individuals seeking plain-vanilla health insurance - a policy that will cover them, say, in case of major surgery or catastrophic illness - may find themselves forced to pay for a policy that also covers acupuncture, in vitro fertilization, alcoholism therapy, and a dozen additional treatments. When compulsion takes the place of competition, the result is invariably less choice at higher cost. (Boston Globe)
Cover yourself Nov 3, 2009
Some benefits, like in vitro fertilization coverage, may sound appealing, but there may not be any real need for it if not many are likely to use it, Gibbs said. Therefore, business owners should survey employees about what benefits are most important to them. (San Jose Business Journal, CA)
Audit finds unacceptable work; Ochsner to keep fertility clinic closed Oct 31, 2009
In September, an embryo mix-up forced Ochsner Health System to suspend its in vitro fertilization program in Elmwood. Quinlan called the announcement "difficult and personal" for Ochsner and its patients. (WWLTV.com, LA)
Soft-Shell Crabs Created On Demand? Oct 31, 2009
SOUNDBITE: Doug Watson, Biologist, University of Alabama at Birmingham IN VITRO, IN THE PETRI DISH, THE RECEPTOR BLOCKER BLOCKS THE EFFECT OF THE MOLT INHIBITING HORMONE, SO WHAT WE WANT TO DO NOW IS TEST IT IN CRABS AND SEE IF IT WILL INDUCE MOLTING IN CRABS.. According to Monterey Bay Aquariums Seafood Watch, a sustainable farming program would need to follow, among other things, strict guidelines surrounding the impact on the wild population. (National Geographic)
Medical Device Maker Rides Chinese Health Care Spending Spree Oct 31, 2009
More recently, it started building an in vitro diagnostics line ... Mindray Medical started out selling patient-monitoring devices in China, then expanded to in vitro diagnostics and has moved into the U.S. market. (Investors Business Daily)
Laying Still Raises Artificial Insemination Success Oct 31, 2009
It differs from in vitro fertilization, which involves fertilizing the egg in a test tube before implanting the embryo in the uterus ... A main downside is a pregnancy rate lower than that achieved by in vitro fertilization. (MEDLINEplus)
Combating infertility problems Oct 31, 2009
Talking about modern techniques that are helping men with low sperm count achieve parenthood, Dr Sohani Verma, IVF in-charge at Indraprastha Apollo Hospitals, says that with modern ART using intra-uterine insemination , in vitro fertilisation and ICSI, couples with male infertility can be helped. "Even when there is no sperm in the semen sample, these can be retrieved from the testes using testicular sperm aspiration or testicular sperm extraction techniques, and thus pregnancy can be achieved.... (India Times, India)
Rationing Care at the Beginning of Life? Oct 30, 2009
The youngest surviving baby born in the United States was Amillia Taylor, born in 2006 after 21 weeks and six days, but as it was noted at the time, because she was conceived via in vitro fertilization, her actual age was closer to 23 weeks. But doctors say making determinations of age and viability are not an exact science. (ABC News)
Epilepsy Drugs Could Treat Alzheimer's And Parkinson's Oct 30, 2009
Bao's team established cell culture models to directly test whether these drugs could preserve neurons in long- and short-term cultures in vitro. They found that neurons showed an increase in viability after treatment with either L-type or T-type calcium channel inhibitors. (Science Daily)
Michigan panel debates stem cell research bills Oct 30, 2009
Facilities that handle in vitro fertilizations also would have to file reports with state health officials detailing how many embryos are created, implanted and donated for research. Associated Press reporter David N. Goodman in Detroit contributed to this story. (Ionia Sentinel-Standard, MI)
Drug-radiation Combo Eradicates Lung Cancer In Mice Oct 30, 2009
Dr. Scaglioni and his team first tested the effectiveness of BEZ235 alone and found that it inhibits the proliferation of both lung cancer cells cultured in vitro and the growth of lung-cancer tumors in mice. "The results were striking, but we wanted to find a strategy to precipitate cell death of these tumors," said Dr. Georgia Konstantinidou, a postdoctoral researcher at UT Southwestern and the lead author of the study. (Science Daily)
1 embryo as good as 2 for in vitro pregnancy chances Oct 29, 2009
BOSTON - Women who initially receive just one embryo during in vitro fertilization are as likely to produce a child as women implanted with two, Swedish researchers reported on Wednesday. The research, which follows up on a 2004 study, comes amid widespread concern over health risks to mothers and babies associated with multiple births that often occur when more than one embryo is implanted using test tube baby technology. (MSNBC -- Health)
Scientists make cells that form eggs, sperm Oct 29, 2009
She said these cells formed a round spermatid, an immature sperm cell that contains just one copy of the chromosomes that would be suitable for use in an in vitro fertilization clinic. Producing too few germ cells or poor quality germ cells is a major cause of infertility in humans. (MSNBC -- Health)
BABIES WITHOUT MEN OR WOMEN Oct 29, 2009
all these same rantings about 'playing god' and brave new worlds of mutant children were made at the advent of in vitro fertilization and frozen embryos. now, they are common practice for those needing assistance to reproduce. (The Drudge Report)
Staphylococcus aureus biofilm formation at the physiologic glucose concentration depends on the S. aureus lineage Oct 29, 2009
For this reason, in vitro biofilm formation of 228 clinical S. aureus isolates of distinct clonal lineages was investigated ... The present study reveals that the MLST CC8 associated genetic background was a predisposing factor for strong biofilm formation in vitro, under all tested glucose concentrations. (BioMed Central)
Lowering the glycemic index of white bread using a white bean extract Oct 28, 2009
Phase 2 has been shown to inhibit alpha-amylase, the complex carbohydrate digesting enzyme, in vitro. The inhibition of alpha-amylase may result in the lowering of the effective Glycemic Index (GI) of certain foods. (Nutrition Journal)
Impotent husband allows father to rape wife for child Oct 27, 2009
When she confronted her in-laws, they accepted that Dharmesh had a medical problem and suggested in vitro fertilisation (IVF). The woman alleged that one day when she was alone, Vimal gave her sleeping tablets and raped her while she was unconscious. (India Times, India)
IVF Insurance Coverage Yields Fewer Multiple Births, Researchers Find Oct 24, 2009
23, 2009) The proportion of in vitro fertilization (IVF) multiple births was lower in the eight states that provide insurance coverage for couples seeking IVF treatment, primarily due to fewer embryos transferred per cycle, Yale School of Medicine researchers report in an abstract presented at the American Society for Reproductive Medicine meeting in Atlanta, Georgia, October 17-21 ... 24, 2009) The common belief is that it won't hurt to try alternative fertility treatments before reverting to... (Science Daily)
Assessing Embryo Metabolism May Aid In Vitro Fertilization (IVF) Success Oct 24, 2009
THURSDAY, Oct. 22 (HealthDay News) -- Examining the metabolism of embryos may help determine which ones offer the best chance of success with in vitro fertilization (IVF), a new study finds. Currently, the process of selecting embryos for implantation in the mother's womb is highly subjective. (MEDLINEplus)
Money Woes Keeping Many Couples from Fertility Treatments Oct 24, 2009
A single cycle of in vitro fertilization (IVF) costs about $12,000, and several cycles may be needed, according to the ASRM. ... Of those, about 27 percent decided not to pursue treatments including in vitro fertilization, in which the egg and sperm are joined outside the womb and then the embryo is placed back into the mother. (MEDLINEplus)
Damaging Inflammatory Response Could Hinder Spinal Cord Repair Oct 23, 2009
They created in vitro models -- essentially, test tube experiments -- in which they examined the effects of M1 and M2 macrophages on neurons, the cells that make up most of the spinal cord and brain ... "The only benefits we've shown so far were in vitro," he said. (Science Daily)
Simple modeling of FtsZ polymers on flat and curved surfaces: correlation with experimental in vitro observations Oct 23, 2009
In vitro, FtsZ polymerizes in a GTP-dependent manner forming a variety of filamentous flexible structures. Based on data derived from the measurement of the in vitro polymerization of Escherichia coli FtsZ cell division protein we have formulated a model in which the fine balance between curvature, flexibility and lateral interactions accounts for structural and dynamic properties of the FtsZ polymers observed with AFM. The experimental results have been used by the model to calibrate the... (BioMed Central)
Scientists: Womb Transplants Could Happen in 2 Years Oct 23, 2009
Women with a transplanted uterus would have to undergo in vitro fertilization treatment to achieve pregnancy and give birth by Caesarean section in order to avoid complications such as ectopic pregnancy. A successful transplant would also be temporary, to avoid the patient having to tak 00003BB5 e immunosuppressant drugs for life, in order to avoid rejection of the womb. (Fox News)
Nanomagnets Guide Stem Cells To Damaged Tissue Oct 23, 2009
The team also demonstrated a six-fold increase in cell capture in an in vitro flow system (where microscopic particles are suspended in a stream of fluid and examined to see how they behave). Although magnetic fields have been used to guide cellular therapies, this is the first time cells have been targeted using a method directly applicable to clinical practice. (Science Daily)
Identifying Metabolism Of Healthy Embryo Could Improve Infertility Treatment Oct 23, 2009
22, 2009) Embryos that are most likely to result in a pregnancy are crucial to the success of in vitro fertilization (IVF) but are difficult to identify ... Embryos that are most likely to result in a pregnancy are crucial to the success of in vitro fertilization (IVF) but are difficult to identify ... 11, 2008) A review of seven clinical trials of acupuncture given with embryo transfer in women undergoing in vitro fertilization (IVF) suggests that acupuncture may improve rates of pregnancy. (Science Daily)
X-Linked Thrombophilia with a Mutant Factor IX (Factor IX Padua) Oct 22, 2009
In vitro, recombinant factor IX R338L had a specific activity that was 5 to 10 times as high as that in the recombinant wild-type factor IX. The R338 substitution causes a gain-of-function mutation, resulting in factor IX that is hyperfunctional. Source Information. (New England Journal of Medicine)
New IVF Guidelines Aim to Reduce Multiple Births Oct 22, 2009
TUESDAY, Oct. 20 (HealthDay News) -- In an effort to reduce multiple births following fertility treatment, the American Society for Reproductive Medicine has revised its recommendations on the number of embryos that should be transferred during in vitro fertilization procedures. The society reports that the guidelines are now different in two major ways. (MEDLINEplus)
Scientists Develop Novel Method To Generate Functional Hepatocytes For Drug Testing Oct 21, 2009
The study, led by the University of Edinburgh's MRC Centre for Regenerative Medicine, paves the way for the creation of a stem cell library that can be used for in vitro hepatic disease models ... The in vitro derived hepatocytes showed similar attributes to PHH cells used for predictive drug toxicology assays, including CYP3A4 and CYP1A2 metabolism. (Science Daily)
Tips from the American Journal of Pathology Oct 21, 2009
Doi Y, Mizuno T, Maki Y, Jin S, Mizoguchi H, Ikeyama M, Doi M, Michikawa M, Takeuchi H, Suzumura A: Microglia activated with the toll-like receptor 9 ligand CpG attenuate oligomeric amyloid ; neurotoxicity in in vitro and in vivo models of Alzheimer's disease. Am J Pathol 2009, 175: 2115-2126. (EurekAlert!)
PETA's push for changes in USDA testing pays off for animals Oct 20, 2009
Specifically, PETA asked the CVB to follow the lead of Europe in adopting an in vitro test for the vaccine for erysipelasan infectious bacterial disease that strikes pigs. Prior to this change, erysipelas vaccine tests had required that pigs be deliberately infected with the disease. (EurekAlert!)
Going out on a limb Oct 20, 2009
Until now in vitro results on bone have been good, and some basic unpublished results from animal models have shown excellent promise for bone regeneration, says Prof. Zilberman. (EurekAlert!)
Celeb Moms in Their 40s Oct 20, 2009
Courteney Cox: In Vitro for Second Baby - ABC News ... Cox, who conceived Coco through in vitro fertilization or IVF, when she was 39, will most likely find it more difficult her second go-round ... "In vitro is a wonderful thing that people can do in this day and age, and I'm lucky enough to be able to afford it," Cox told Good Housekeeping. (ABC News)
'Octomom' doctor expelled from fertility group Oct 19, 2009
Suleman already had six children, all conceived via in vitro fertilization, in which eggs were taken from her ovaries and combined with donor sperm in a petri dish. Suleman has said that her fertility doctor, Michael Kamrava of the West Coast IVF Clinic in Beverly Hills, transferred six embryos to her uterus for each of her pregnancies. (USA Today -- News)
Stanford study identifies cellular mechanism that causes lupuslike symptoms in mice Oct 19, 2009
Mukundan, Chawla and their colleagues have uncovered one of the pathways by which macrophages sense and silently dispose of these dying cells a naturally-occurring process not previously understood by conducting lab experiments in vitro with mouse and human macrophages, as well as in genetically engineered mice. Researchers hypothesized that a molecule in the nucleus of cells called PPAR-delta plays a pivotal role in orchestrating the timely disposal of dying cells by macrophages, the white... (EurekAlert!)
Fate Therapeutics announces creation of small molecule platform for commercial-scale reprogramming Oct 19, 2009
The iPSC colonies generated by the Ding team using a 3-compound cocktail could be stably expanded over the long term (20+ passages), closely resembled human embryonic stem cells in terms of morphology and pluripotency marker expression and could be differentiated into derivatives of all the three germ layers both in vitro and in vivo. "Once we achieved reprogramming with cell-penetrating proteins, we targeted certain biological pathways that might improve speed and efficiency so as to enable the... (EurekAlert!)
Stem Cells Grow Heart Tissue in Lab Oct 17, 2009
"The usual progression of these things is from in vitro [laboratory] work, then small animal models using human cells, then to larger animals, such as pigs," Marban said. "It took us five years to a first human trial, and that was a wildly aggressive schedule. Ten years for them would be a remarkable achievement.". (MEDLINEplus)
Can Black Elderberry Extract Preven... Oct 17, 2009
A recent study published in the July 2009 issue of the scientific journal, Phytochemistry (2009 Jul;70(10):1255-61), declares that "elderberry flavonoids bind to and prevent H1N1 infection in vitro." Similarly, a study published in the Journal of International Medical Research (2004 Mar-Apr;32(2):132-40) examined the efficacy of black elderberry syrup (marketed under the name Sambucol) in a group of flu sufferers in Norway and determined that their symptoms were relieved on average 4 days... (Suite101.com)
Honey as a Wound Therapy Oct 16, 2009
Journal of Wound Care, Du Toit and Page, An in vitro evaluation of the cell toxicity of honey and silver dressings, September 2009. Epidemiologie, mikrobiologie, imunologie, Majtan, Apitherapy--the role of honey in the chronic wound healing process, August 2009. (Suite101.com)
Abortion foes challenge pay for egg donors Oct 15, 2009
When the state Stem Cell Board approved the payments, the group posted a statement noting that compensation is allowed for women who donate eggs for reproductive purposes, such as in vitro fertilization. "There is no principled reason to distinguish between donation of oocytes for reproductive purposes and research purposes when determining the ethicality of reimbursement," the board wrote. (Albany Times Union)
Evotec Extends Medicinal Chemistry Collaboration With Ono Pharmaceutical Co., Ltd. Oct 15, 2009
Under the contract extension Evotec will provide integrated drug discovery activities, including high throughput screening, medicinal chemistry and in vitro pharmacology to discover novel, small molecular weight compounds active against an ion channel target selected by Ono. Ono will access Evotec's ion channel drug discovery platform and expertise and Evotec will receive research funding and milestone payments. (Primezone Releases)
OTHER OPINION: RESEARCH Oct 15, 2009
Usually, couples who go to in vitro fertilization clinics create more embryos than will be implanted. The remainder are either destroyed or frozen. (Brainerd Daily Dispatch)
LETTER: People have many choices of protection Oct 15, 2009
"""""these do-gooders are the same ones who will use an in vitro clinic and use only half of their embryos. those left are later flushed down a sewer. using their very own logic, this is abortion, and they are baby killers. ". The Question wrote on Oct 14, 2009 5:42 PM:" You know, it must be frustrating to be a fundamentalist, always being proven so glaringly wrong about everything, from Galileo to Scopes, and constantly being made to look foolish by the very nature of reality. How strange it... (Mattoon Journal-Gazette, IL)
Statement In Response To Omitted Child Petition Action for Mrs. Sherri Crichton Oct 14, 2009
In June 2008, Michael and Sherri each underwent fertility procedures at the Center for Reproductive Medicine and Infertility at the New York Presbyterian Hospital -Weill Medical College of Cornell University to become pregnant with their son, John Michael, by in vitro fertilization. In an effort to keep this private family matter out of the public eye, when Taylor Crichton demanded proof of her brother's paternity in connection with the legal proceeding to determine John Michael's right to... (Yahoo! Wire -- Entertainment News)
